Sequence-Dependent Enhancement of Cy3 Fluorescence on DNA
نویسندگان
چکیده
منابع مشابه
Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled Double-Stranded DNA
The fluorescent intensity of Cy3 and Cy5 dyes is strongly dependent on the nucleobase sequence of the labeled oligonucleotides. Sequence-dependent fluorescence may significantly influence the data obtained from many common experimental methods based on fluorescence detection of nucleic acids, such as sequencing, PCR, FRET, and FISH. To quantify sequence dependent fluorescence, we have measured ...
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Cyanine dyes are commonly used for fluorescent labeling of DNA and RNA oligonucleotides in applications including qPCR, sequencing, fluorescence in situ hybridization, Förster resonance energy transfer, and labeling for microarray hybridization. Previous research has shown that the fluorescence efficiency of Cy3 and Cy5, covalently attached to the 5' end of single-stranded DNA, is strongly sequ...
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DNA sequence-dependent photoluminescence enhancement is found for a cationic polyelectrolyte complexed with single stranded DNA and described as a result of an interplay between electrostatic attraction and the π-π stacking between the polyelectrolyte's backbone and DNA's bases.
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We report the distance-dependent fluorescence behavior of Au-DNA conjugates prepared by equilibrating the phosphine-stabilized gold nanoparticles (AuNPs) of 10-nm size with the designed rigid spacer double-stranded DNA consisting of thiol-modified target and Cy3-labelled complementary probe of different lengths (5 to 20 nm). The products were immobilized onto a MPTMS (3-mercaptopropyltrimethoxy...
متن کاملSequence - dependent on formation of DNA Duplexes
The nonexchangeable base and sugar protons of the octanucleotide d(G-G-A-A-T-T-C-C) have been assigned by two-dimensional correlated (COSY) and nuclear Overhauser effect (NOESY) methods in aqueous solution. The assignments are based on distance connectivities of 4 . 5 A established from NOE effects between base and sugar protons on the same strand and occasionally between strands, aswell as,...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2010
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2009.12.3161