Sequence diversity and evolution of infectious bursal disease virus in Iraq

Sequence analysis of the VP1 gene in three very virulent Iranian infectious bursal disease virus strains

Infectious bursal disease (IBD) is a highly contagious disease of chickens caused by the infectious bursal disease virus (IBDV). This study was conducted to characterize three IBDV strains from Iran. A reverse transcriptase-polymerase chain reaction (RT-PCR) procedure was used to amplify a 715-bp fragment of the VP1 gene from IBDV strains. Amplified VP1 fragments of the three Iranian IBDV strai...

Characterization of a Highly Virulent Infectious Bursal Disease Virus

Genome Sequence of a Distinct Infectious Bursal Disease Virus

Infectious bursal disease virus is a relevant avian pathogen that affects poultry production. Here, we report the full-length coding sequence of the Uruguayan strain dIBDV/UY/2014/2202, isolated from a commercial broiler flock. The strain belongs to the distinct IBDV lineage that is widely distributed in South America.

Cloning and secretory expression of VP2 gene of infectious bursal disease virus in eukaryotic cells

VP2 gene coding region of a vaccinal strain (D78) of infectious bursal disease virus (IBDV) was clonedin a eukaryotic expression vector, pSec Tag2A. The gene was placed downstream of Ig κ chain leadersequence, under the control of human cytomegalovirus (hCMV) immediate early enhancer and promoter. Theconstruct pSec Tag2A-VP2 was transfected in COS-7 cell line and the expression and secretion of...

Cloning and Expression of VP2 Gene of Infectious Bursal Disease Virus in Eukaryotic Cells

Infectious bursal disease (IBD) is an economically important viral disease of chickens with worldwide distribution which suppresses the immune system of young chickens. VP2 is the major host-protective protein of infectious bursal disease virus (IBDV). The encoding region of VP2 protein was PCR amplified from a plasmid containing a cDNA fragment of large genomic segment of IBDV, strain D78. Thi...