Sequence-specific responses of restriction endonucleases to bromodeoxyuridine substitution in mammalian DNA
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چکیده
منابع مشابه
Sequence-specific responses of restriction endonucleases to bromodeoxyuridine substitution in mammalian DNA.
Substitution of BrdU for dT in mammalian DNA alters the rates of DNA cleavage by restriction endonucleases in a manner that can be related to the specificity of cleavage. A formula is proposed that describes inhibitory and stimulatory contributions arising from the substitution of a Br atom for the CH3 group on T. The larger Br atom is postulated to sterically hinder the nuclease from binding t...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1983
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/11.8.2495