Sequencing artifacts derived from a library preparation method using enzymatic fragmentation
نویسندگان
چکیده
منابع مشابه
Correction: Palindromic Sequence Artifacts Generated during Next Generation Sequencing Library Preparation from Historic and Ancient DNA
Degradation-specific processes and variation in laboratory protocols can bias the DNA sequence composition from samples of ancient or historic origin. Here, we identify a novel artifact in sequences from historic samples of Atlantic cod (Gadus morhua), which forms interrupted palindromes consisting of reverse complementary sequence at the 5' and 3'-ends of sequencing reads. The palindromic sequ...
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A: Damage can be limited by performing acoustic shearing of genomic DNA in 1X TE (10 mM Tris pH 8, 1 mM EDTA), rather than less buffered solutions. Treating the genomic DNA with the NEBNext FFPE DNA Repair Mix after shearing, but before library preparation, will also reduce DNA damage prior to sequencing. More information about DNA damage and repair mixes can be found on our website. Additional...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2020
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0227427