Sequential Ultrastructural Changes of WISH Cells Infected with Encephalomyocarditis Virus

Virus-specific proteins synthesized in encephalomyocarditis virus-infected HeLa cells.

The in vivo synthesis of encephalomyocarditis-specific proteins was studied by labeling the viral proteins with radioactive amino acids under conditions where host-protein synthesis was almost completely inhibited. To assure recovery of all proteins, intact cells were lysed in hot 1% sodium dodecyl sulfate. These lysates were analyzed by quantitative high-resolution electrophoresis on sodium do...

Macromolecular synthesis in cells infected with Herpes simplex virus

Sequential cellular changes produced by african horse sickness virus in monkey kidney cells

Sites of Synthesis of Encephalomyocarditis Virus Components in Infected L-cells.

L-cells were fractionated at various times after infection with EMC virus and assayed for haemagglutinin, virus and phenol-extractable infective RNA. A second fraction of infective RNA was obtained by sodium dodecyl sulphate (SDS) extraction of the samples after phenol extraction. The infectivity of SDS-extracted RNA increased earlier than that of phenol-extracted RNA, but the maximum titre was...

Interaction of encephalomyocarditis virus with ultraviolet-irradiated host cells.

Ultraviolet light (UV) impaired the capacity of L cells to support growth of encephalomyocarditis virus. The loss of capacity was partially restored by high multiplicity of infection (MOI). This phenomenon was not due to an increased probability of an infectious virus particle reaching a site of replication undamaged by UV, since UV-inactivated virus at high MOI induced restoration of the capac...