Serological Detection of Citrus tristeza virus with Antibodies Developed to the Recombinant Coat Protein
نویسندگان
چکیده
منابع مشابه
Serological Detection of Grapevine leafroll virus 2 Using an Antiserum Developed against the Recombinant Coat Protein
Grapevine leafroll associated virus 2 (GLRaV 2) is one of the important components in the leafroll disease complex. The coat protein gene of GLRaV 2 was cloned into a protein expression vector pMAL-c2x and the recombinant protein, consisting of the maltose binding protein (MBP) and GLRaV 2 coat protein (CP), was expressed in Escherichia coli. The recombinant MBP-CP was used to raise a high qual...
متن کاملBacterial expressed coat protein: development of a single antiserum for routine detection of Citrus Tristeza Virus
Citrus tristeza virus (CTV, genus Closterovirus, family Closteroviridae) is the largest member of its taxonomic family. Viral particles, 2000 nm in length and 10–12 nm in diameter, are present in the phloem of infected plants. A single stranded positive sense RNA molecule approximately 20,000 nucleotides long constitutes its genome (BarBacterial expressed coat protein: development of a single a...
متن کاملExpression of Citrus tristeza virus coat protein gene in Escherichia coli
Citrus tristeza virus (CTV) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. Considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in Iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in Iran, as one of the major citr...
متن کاملCitrus tristeza virus-host interactions
Citrus tristeza virus (CTV) is a phloem-limited virus whose natural host range is restricted to citrus and related species. Although the virus has killed millions of trees, almost destroying whole industries, and continually limits production in many citrus growing areas, most isolates are mild or symptomless in most of their host range. There is little understanding of how the virus causes sev...
متن کاملexpression of citrus tristeza virus coat protein gene in escherichia coli
citrus tristeza virus (ctv) is among the most destructive pathogens of citrus and causes substantial economic losses in citrus-growing industry worldwide. considering recent distribution of this pathogen and its capability of transmission by existing aphid vectors in iran, detection of this virus is enforceable for controlling the damage caused by this pathogen in iran, as one of the major citr...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 2009
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis-93-1-0011