Serotyping Streptococcus pneumoniae by Multiplex PCR


برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Serotyping Streptococcus pneumoniae by multiplex PCR.

The capsule is a major virulence factor of pneumococci, and it was shown that some capsular variants are associated with antimicrobial resistance and certain types of disease. Moreover, pneumococcal capsular typing has received renewed interest since the availability of conjugate vaccines, which include serotypes frequently associated with pediatric disease. Our aim was to develop a simple, rel...

متن کامل

Sequetyping: serotyping Streptococcus pneumoniae by a single PCR sequencing strategy.

The introduction of pneumococcal conjugate vaccines necessitates continued monitoring of circulating strains to assess vaccine efficacy and replacement serotypes. Conventional serological methods are costly, labor-intensive, and prone to misidentification, while current DNA-based methods have limited serotype coverage requiring multiple PCR primers. In this study, a computer algorithm was devel...

متن کامل

Efficacy of multiplex PCR procedure for Iranian Streptococcus pneumoniae isolates

Background: Identifying regional types and evaluating the frequency of pneumococcal strains has become increasingly important especially in vaccination. The purpose of this study was the identification and frequency determination of our regional serotype and evaluation of the performance of recent type specific multiplex PCR for the diagnosis of streptococcus pneumonia serotypes. Methods: All ...

متن کامل

Comparison of sequential multiplex PCR, sequetyping and whole genome sequencing for serotyping of Streptococcus pneumoniae

Streptococcus pneumoniae is one of the major causes of pneumonia, meningitis and other pneumococcal infections in young children and elders. Determination of circulating S. pneumoniae serotypes is an essential service by public health laboratories for the monitoring of putative serotype replacement following the introduction of pneumococcal conjugate vaccines (PCVs) and of the efficacy of the i...

متن کامل

Serotyping of Streptococcus pneumoniae strains by coagglutination

The coagglutination test, which uses staphylococcal protein A, for serotyping strains of Streptococcus pneumoniae, was extended to include serotyping within serogroups. Serotyping was performed with "factor sera" prepared in the laboratory. Fifty one strains of S pneumoniae, which belonged to one of the seven serogroups included in the 14 valent vaccine formulation, were tested, and no inconsis...

متن کامل

ذخیره در منابع من

  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال: Journal of Clinical Microbiology

سال: 2003

ISSN: 0095-1137,1098-660X

DOI: 10.1128/jcm.41.6.2378-2384.2003