Short donor site sequences inserted within the intron of beta-globin pre-mRNA serve for splicing in vitro.
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چکیده
منابع مشابه
Short donor site sequences inserted within the intron of beta-globin pre-mRNA serve for splicing in vitro.
We constructed SP6-human beta-globin derivative plasmids that included possible donor site (5' splice site) sequences at a specified position within the first intron. The runoff transcripts from these templates truncated in the second exon were examined for splicing in a nuclear extract from HeLa cells. In addition to the products from the authentic donor site, a corresponding set of novel prod...
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The rat beta-tropomyosin (beta-TM) gene encodes both skeletal muscle beta-TM mRNA and nonmuscle TM-1 mRNA via alternative RNA splicing. This gene contains eleven exons: exons 1-5, 8, and 9 are common to both mRNAs; exons 6 and 11 are used in fibroblasts as well as in smooth muscle, whereas exons 7 and 10 are used in skeletal muscle. Previously we demonstrated that utilization of the 3' splice s...
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متن کاملMechanism for cryptic splice site activation during pre-mRNA splicing.
The 5' splice site of a pre-mRNA is recognized by U1 small nuclear ribonucleoprotein particles (snRNP) through base pairing with the 5' end of U1 small nuclear RNA (snRNA). Single-base substitutions within a 9-nucleotide 5'-splice-site sequence can abolish or attenuate use of that site and, in higher eukaryotes, can also activate nearby "cryptic" 5' splice sites. Here we show that the effects o...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1988
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.8.10.4484