Simple and efficient protocol for immunoglobulin Y purification from chicken egg yolk
نویسندگان
چکیده
منابع مشابه
a simple and economical procedure for the purification of immunoglobulin y from egg yolk by t-gel chromatography
the use of immunoglobulin y (igy) instead of polyclonal antibodies prepared in mammals has been reported recently. these antibodies are found to have better properties in terms of specificity and ease of large scale production. in this study, proteins from egg yolk were extracted with acid and chloroform. the result of cellulose acetate electrophoresis at ph 9 showed some extra bands which were...
متن کاملSequential separation of immunoglobulin Y and phosvitin from chicken egg yolk without using organic solvents.
A study was conducted to develop a simple sequential separation protocol to separate phosvitin and IgY from egg yolk without using organic solvents. Egg yolk was diluted with 2 volumes of distilled water (DW), homogenized, and centrifuged. The precipitant was collected and homogenized with 4 volumes of 10% NaCl (wt/vol) in 0.05 N NaOH solution to extract phosvitin. The pH of the homogenate was ...
متن کاملA simple method for isolating chicken egg yolk immunoglobulin using effective delipidation solution and ammonium sulfate.
Chicken egg yolk immunoglobulin (IgY) is a superior alternative to mammalian immunoglobulin. However, the practical application of IgY in research, diagnostics, and functional food is limited due to complex or time-consuming purification procedures. The objective of this study was to develop a simple, safe, large-scale separation method for IgY from egg yolk. Egg yolk was diluted with 6-fold de...
متن کاملidentification and extraction of chicken egg yolk immunoglobulin from egg by polyethylene glycol (peg) precipitation
background : staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. treatment of patients with these infections has been difficult. the aim of this study was to detect and extract, the egg yolk immunoglobulin y as a potential source of anti- s. aureus antibody. methods : specific igy was produced by immunizing hens wi...
متن کاملComparison of Four Different Purification Methods for Isolation of Anti Echis carinatus Antivenom Antibodies from Immunized Chicken Egg Yolk
Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 2021
ISSN: 0032-5791
DOI: 10.1016/j.psj.2020.12.053