Simple control of fed-batch processes for recombinant protein production with E. coli

Optimisation Methods for Improving Fed-batch Cultivation of E. Coli Producing Recombinant Proteins

Two optimisation techniques for the fed-batch cultivation of high cell density Escherichia coli producing recombinant proteins were compared. An unstructured model for the growth, based on the General State Space Dynamical Model [1] was used to represent the four major metabolic pathways: oxidative growth on glucose, fermentative growth on glucose, oxidative growth on acetate, and maintenance. ...

Monitoring of fed-batch E. coli fermentations with software sensors.

Accurate monitoring and control of industrial bioprocess requires the knowledge of a great number of variables, being some of them not measurable with standard devices. To overcome this difficulty, software sensors can be used for on-line estimation of those variables and, therefore, its development is of paramount importance. An Asymptotic Observer was used for monitoring Escherichia coli fed-...

Design of On-line State Estimators for a Recombinant E. Coli Fed-batch Fermentation

In recent years a remarkable effort has been made in the development of new sensors and process analytical technology. However, it is still difficult to find reliable and low cost commercial sensors for on-line measurements of important variables. Therefore, considerable attention has been focused on the development of on-line software sensors. Nevertheless, the application of those algorithms ...

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

Optimal Feeding Trajectories Design for E. coli Fed-batch Fermentations

In this paper optimal control algorithms for two E. coli fed-batch fermentations are developed. Fed-batch fermentation processes of E. coli strain MC4110 and E. coli strain BL21(DE3)pPhyt109 are considered. Simple material balance models are used to describe the E. coli fermentation processes. The optimal feed rate control of a primary metabolite process is studied and a biomass production is u...