Simple technique to identify haemosiderin in immunoperoxidase stained sections.

Human neurospHeres : from stained sections to tHree - dimensional assembly

Human neurospheres are free-floating spherical clusters generated from a single neural stem cell and comprising cells at different stages of maturation in the neuronal and glial lineages. Although recent findings have disproved the original idea of clonally derived neurospheres according to the paradigm of one stem cell – one neurosphere, they still represent a valid model for growing neural st...

Direct immunoperoxidase method for demonstrating Chlamydia psittaci in tissue sections.

Plastination of stained sections of the human brain.

A method is described which combines staining of human brain tissue with subsequent plastination of the sections. Staining of 1-4 mm thick frozen sections with astra blue or aldehydefuchsin provides a sharp contrast between white and grey matter. The plastination results in sections which are durable and convenient to handle. These preparations may be used for teaching purposes in neuroanatomy ...

Comparison of Weigert stained sections with unfixed, unstained sections for study of myelin sheaths.

Myelinated fibers can be demonstrated in thin unstained brain sections of freshly killed uninjected animals. With this method myelinated fibers stand out as clearly as in stained Weigert sections from formalin-fixed brains. Unstained unfixed sections can be viewed under ordinary light within minutes after death of the animal and immediately photographed for permanent records. Such sections have...

Immunoperoxidase technique in histopathology: applications, methods, and controls.