Simultaneous analysis of cell surface antigens, bromodeoxyuridine incorporation and DNA content
نویسندگان
چکیده
منابع مشابه
Simultaneous Camouflage of Major and Minor Antigens on Red Blood Cell Surface With Activated mPEGs
Background: Host immune system response against blood group antigens is a major problem in blood transfusions, especially for <span style="font-varian...
متن کاملDifferent effects of bromodeoxyuridine and [3H]thymidine incorporation into DNA on cell proliferation, position, and fate.
As markers of DNA synthesis, [(3)H]thymidine ([(3)H]dT) and the later developed analog bromodeoxyuridine (BrdU) have revolutionized our ability to identify dividing cells and follow their fate in various tissues, including the nervous system. However, the effect of the incorporation of these molecules into DNA on cell proliferation, migration, differentiation, and function is not fully understo...
متن کاملDRAQ5-based DNA content analysis of hematolymphoid cell subpopulations discriminated by surface antigens and light scatter properties.
BACKGROUND Analysis of cell cycle kinetics offers important information regarding the behavior of normal and neoplastic cells. Most often, cell cycle determinations by flow cytometry (FCM) have been performed using whole-sample analysis with intercalating dyes like propidium iodide (PI). The cell cycle phase assessment in individual cell subsets in heterogeneous samples is best performed using ...
متن کاملsimultaneous camouflage of major and minor antigens on red blood cell surface with activated mpegs
background: host immune system response against blood group antigens is a major problem in blood transfusions, especially forthalassemic patients. thus, an approach was proposed coating the red blood cell (rbc) surface by polyethylene glycol.objectives: this study aimed to obtain the optimal simultaneous camouflge of the major and minor antigens by activated methoxypolyethylene glycol (mpeg) wi...
متن کاملSimultaneous analysis of DNA and cell surface immunoglobulin in human B-cell lymphomas by flow cytometry.
Cell suspensions prepared from human B-cell lymphomas were analyzed for cellular DNA and cell surface immunoglobulin (SIg) by flow cytometry. In addition to SIg-bearing neoplastic B-lymphocytes, these tumors contain nonneoplastic cells. Flow cytometry permitted selective analysis of DNA in subpopulations defined by the membrane antigen SIg and also permitted analysis of SIg of subpopulations de...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1985
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990060607