Simultaneous quantification of oxidative stress and cell spreading using 5-(and-6)-chloromethyl-2′,7′-dichlorofluorescein
نویسندگان
چکیده
منابع مشابه
Quantifying cellular oxidative stress by dichlorofluorescein assay using microplate reader.
Oxidative stress (OS) has been implicated in various degenerative diseases in aging. In an attempt to quantify OS in a cell model, we examined OS induced by incubating for 30 min with various free radical generators in PC12 cells by using the dichlorofluorescein (DCF) assay, modified for use by a fluorescent microplate reader. The nonfluorescent fluorescin derivatives (dichlorofluorescin, DCFH)...
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ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2006
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.20348