Site-Directed <em>φC31</em>-Mediated Integration and Cassette Exchange in <em>Anopheles</em> Vectors of Malaria
نویسندگان
چکیده
منابع مشابه
Production of viral vectors using recombinase-mediated cassette exchange
DNA viruses are often used as vectors for foreign gene expression, but large DNA region from cloned or authentic viral genomes must usually be handled to generate viral vectors. Here, we present a unique system for generating adenoviral vectors by directly substituting a gene of interest in a small transfected plasmid with a replaced gene in a replicating viral genome in Cre-expressing 293 cell...
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A targeting method to insert genes at a previously characterized genetic locus to make plant transformation and transgene expression predictable is highly desirable for plant biotechnology. We report the successful targeting of transgenes to predefined soybean (Glycine max) genome sites using the yeast FLP-FRT recombination system. First, a target DNA containing a pair of incompatible FRT sites...
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BACKGROUND Adenovirus serotype 5 (Ad5) has many favourable characteristics for development as a gene therapy vector. However, the utility of current Ad5 vectors is limited by transient transgene expression, toxicity and immunogenicity. The most promising form of vector is the high capacity type, which is deleted for all viral genes. However, these vectors can only be produced to relatively low ...
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Background & Aims of the Study: The aim of this study was to determine the ecology of malaria vectors in order to select the vector control masseur in the future. This study was carried out on trend of malaria cases and malaria vectors in Saravan county, Sistan & Baluchestan Province, Iran. Materials & Methods: The adult mosquitoes were collected by standard Pyrethrum space spray colle...
متن کاملSite-specific bacterial chromosome engineering: ΦC31 integrase mediated cassette exchange (IMCE).
The bacterial chromosome may be used to stably maintain foreign DNA in the mega-base range. Integration into the chromosome circumvents issues such as plasmid replication, plasmid stability, plasmid incompatibility, and plasmid copy number variance. This method uses the site-specific integrase from the Streptomyces phage (Φ) C31. The ΦC31 integrase catalyzes a direct recombination between two s...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2021
ISSN: 1940-087X
DOI: 10.3791/62146