Size increase of YAC insertions without fractionation by PFGE
نویسندگان
چکیده
منابع مشابه
Yeast colony size reflects YAC copy number.
A novel strategy for separation of co-cloned YACs was developed. For this, yeast cells were grown under non-selective conditions to allow the mitotic loss of multiple YACs. Yeast colonies of different size appear on 'drop-out' selection plates with small clones consistently containing a single-copy YAC. Different auxotrophic marker genes can be used to separate co-cloned YACs or reduce their co...
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ژورنال
عنوان ژورنال: Technical Tips Online
سال: 1998
ISSN: 1366-2120
DOI: 10.1016/s1366-2120(08)70094-1