SNP-based genetic linkage map of tobacco (Nicotiana tabacum L.) using next-generation RAD sequencing
نویسندگان
چکیده
منابع مشابه
Genetic Study of Agronomic Traits in Tobacco (Nicotiana tabacum L.) Using Hayman’s Graphical Analysis of Diallel
For the purpose of graphical analysis and estimation of genetic parameters related to yield and its components, six tobacco genotypes was crossed in half -diallel mating design. In this study, the F2 progenies and their parents were evaluated in a randomized complete block design with three replications. The results of analysis of variance indicated a significant difference between genotypes fo...
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1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2. An apparent K(m) value of 3.6x10(-5)m was foun...
متن کاملBiotechnological Reduction of Tobacco (Nicotiana Tabacum L.) Toxicity
BACKGROUND: Nicotiana tobacco contains large amounts of alkaloid nicotine. Tobacco plant is used for smoking and causes many health problems since it is high in nicotine which is one of the widely-recognized toxic compounds with serious side effects for different body organs. Reducing nicotine content of this plant is a way to reduce its health hazards in cigarette smokers. Utilizing the new ...
متن کاملassessing the genetic diversity of tobacco (nicotiana tabacum l.) varieties
the genetic diversity of 40 tobacco (nicotiana tabacum l.) cultivars was analyzed using 12 issr primers. thecultivars were evaluated for 14 traits using a simple lattice design (7×7) with two replications. of 149 amplified bands, 142 (95.3%) were polymorphic. maximum and minimum bands were generated by primers ubc825 (17 bands and 94.11% polymorphic) and ubc824 (7 bands and 100% polymorphic), r...
متن کاملLinkage Mapping and Comparative Genomics Using Next-Generation RAD Sequencing of a Non-Model Organism
Restriction-site associated DNA (RAD) sequencing is a powerful new method for targeted sequencing across the genomes of many individuals. This approach has broad potential for genetic analysis of non-model organisms including genotype-phenotype association mapping, phylogeography, population genetics and scaffolding genome assemblies through linkage mapping. We constructed a RAD library using g...
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ژورنال
عنوان ژورنال: Journal of Biological Research-Thessaloniki
سال: 2015
ISSN: 2241-5793
DOI: 10.1186/s40709-015-0034-3