Sodium Deoxycholate Micelles Activated Separation of Coexisting Fivenucleobases by High-performance Thin-layer Chromatography

Sodium Deoxycholate Micelles Activated Separation of Coexisting Five- nucleobases by High-performance Thin-layer Chromatography

High-performance thin-layer chromatography (HPTLC) is well suited to the separation of nucleobases. Most of reports on this topic are limited to the use of PEI-cellulose, ODS (octadecyl silica), and silica gel as layer materials (Randerath and Struck, 1961; Bij and Lederer, 1983; Steinberg et al., 1996). Chiral plates have also been used for separation of nucleobases, and enantiomers (Hatzack a...

A preparative high performance liquid chromatography method for separation of lecithin: comparison to thin-layer chromatography.

We have developed a high performance liquid chromatography (HPLC) method to separate lecithin from other phospholipid classes and to obtain lecithin from biologic materials. The separation was performed on a preparative 10-micron Spherisorb column with an optimized solvent system consisting of the following components: acetonitrile, isopropanol, methanol, water, and trifluoroacetic acid. The ad...

Quantitative Estimation of Efavirenz by High Performance Thin Layer Chromatography

A simple, accurate, and precise HPTLC method has been developed and validated for the estimation of efavirenz from bulk drug and capsule formulations. The separation was achieved on TLC plates using an appropriate solvent system. The spots so developed were densometrically scanned at 247 nm. The linearity of the method was found to be within the concentration range of 1-40 μg/ml. The validation...

SEPARATION OF NONHISTONE HIGH MOBILITY GROUP (HMG) FROM HUMAN LYMPHOCYTES BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

The high mobility group (HMG) of nonhistone proteins have been investigated using two high performance liquid chromatographic techniques (HPLC). Reversed-phase HPLC under conditions of 50 mM triethylamine adjusted to pH 2.2 with phosphoric acid (solvent A) and 95% acetonitrile in water (solvent B) was used to separate proteins primarily on the basis of differences in the overall hydrophobi...

Separation of Cholesterol and Demosterol by Thin-layer Chromatography.