Somatic mutation in rearranged IgHV gene of B-NHLs
نویسندگان
چکیده
منابع مشابه
Extensive and selective mutation of a rearranged VH5 gene in human B cell chronic lymphocytic leukemia
B cell chronic lymphocytic leukemia (CLL) is the malignant, monoclonal equivalent of a human CD5+ B cell. Previous studies have shown that the VH and VL genes rearranged and/or expressed in CLL have few and apparently random mutations. However, in this study, we have found that the rearranged VH251 gene, one of the three-membered VH5 family, has extensive and selective mutations in B-CLL cells....
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B-cell clonality analysis is commonly performed by polymerase chain reaction (PCR) targeting the IGH genes although a high false-negative rate is recognized for germinal centre/post-germinal centre B-cell malignancies, especially follicular lymphoma. We assessed the diagnostic value of BIOMED-2 IGK assays and investigated the cause of IGH PCR failure in 77 patients with follicular lymphoma. Usi...
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We performed IGH clonotypic sequence analysis in WM in order to determine whether a preferential IGH gene rearrangement was observed and to assess IGHV mutational status in blood and/or bone marrow samples from 36 WM patients. In addition we investigated the presence of MYD88 L265P somatic mutation. After IGH VDJ locus amplification, monoclonal VDJ rearranged fragments were sequenced and analyz...
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ژورنال
عنوان ژورنال: The journal of the Japanese Society of Lymphoreticular Tissue research
سال: 1999
ISSN: 1883-681X,1342-9248
DOI: 10.3960/jslrt1997.39.259