Somatic mutation landscapes at single-molecule resolution
نویسندگان
چکیده
منابع مشابه
Complex germline and somatic mutation processes at a haploid human minisatellite shown by single-molecule analysis
Mutation at most human minisatellites is driven by complex interallelic processes that give rise to a high degree of length polymorphism and internal structural variation. MSY1, the only highly variable minisatellite on the non-recombining region of the Y chromosome, is constitutively haploid and therefore precluded from interallelic interactions, yet maintains high diversity in both length and...
متن کاملTracking viral genomes in host cells at single-molecule resolution.
Viral DNA trafficking in cells has large impacts on physiology and disease development. Current methods lack the resolution and accuracy to visualize and quantify viral DNA trafficking at single-molecule resolution. We developed a noninvasive protocol for accurate quantification of viral DNA-genome (vDNA) trafficking in single cells. Ethynyl-modified nucleosides were used to metabolically label...
متن کاملResolving Subcellular miRNA Trafficking and Turnover at Single-Molecule Resolution.
Regulation of microRNA (miRNA) localization and stability is critical for their extensive cytoplasmic RNA silencing activity and emerging nuclear functions. Here, we have developed single-molecule fluorescence-based tools to assess the subcellular trafficking, integrity, and activity of miRNAs. We find that seed-matched RNA targets protect miRNAs against degradation and enhance their nuclear re...
متن کاملMutation landscapes.
A traditional picture of evolutionary dynamics with constant fitness is that of genomes living in sequence space and adapting on fitness landscapes. Mutation rates are considered to be constant or externally regulated. If, however, we take into account that genomes also encode for enzymes that perform replication and error correction, then individual genomes not only have a specific replication...
متن کاملExploring protein folding landscapes: from ensembles to single molecule methods
59 KEY TO ABSTRACT NUMBERING
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature
سال: 2021
ISSN: 0028-0836,1476-4687
DOI: 10.1038/s41586-021-03477-4