Spaced seeds improvek-mer-based metagenomic classification

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Spaced seeds improve k-mer-based metagenomic classification

MOTIVATION Metagenomics is a powerful approach to study genetic content of environmental samples, which has been strongly promoted by next-generation sequencing technologies. To cope with massive data involved in modern metagenomic projects, recent tools rely on the analysis of k-mers shared between the read to be classified and sampled reference genomes. RESULTS Within this general framework...

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Metagenomic reads binning with spaced seeds

Article history: Received 23 February 2017 Received in revised form 16 May 2017 Accepted 21 May 2017 Available online xxxx

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Vector seeds: An extension to spaced seeds

We present improved techniques for finding homologous regions in DNA and protein sequences. Our approach focuses on the core regions of a local pairwise alignment; we suggest new ways to characterize these regions that allow marked improvements in both specificity and sensitivity over existing techniques for sequence alignment. For any such characterization, which we call a vector seed, we give...

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Clustering metagenomic reads using spaced k-mers

With the emergence of next-generation sequencing technologies, the classification of short reads in a metagenomic sample has become an important yet difficult task. Several tools attempt to tackle this problem with each having a strong point in certain situations. Herein, a novel method is proposed that has its strong point in processing short reads. It is based on two new concepts: utilizing m...

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A coverage criterion for spaced seeds and its applications to SVM string-kernels and k-mer distances

Spaced seeds have been recently shown to not only detect more alignments, but also to give a more accurate measure of phylogenetic distances (Boden et al., 2013, Horwege et al., 2014, Leimeister et al., 2014), and to provide a lower misclassification rate when used with Support Vector Machines (SVMs) (Onodera and Shibuya, 2013), We confirm by independent experiments these two results, and propo...

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ژورنال

عنوان ژورنال: Bioinformatics

سال: 2015

ISSN: 1367-4803,1460-2059

DOI: 10.1093/bioinformatics/btv419