SPATIAL DISTRIBUTION AND FUNCTIONAL RELEVANCE OF FGFR1 AND FGFR2 EXPRESSION FOR GLIOBLASTOMA TUMOUR INVASION

Abstract AIMS Glioblastoma (GBM) is the most common brain tumour in adults. The GBM microenvironment made up of heterogeneous cell populations, but how microenvironmental signalling pathways contribute to het- erogeneity and specific pathological features remains incompletely understood. Here, we compare expression fibroblast growth factor receptors (FGFRs) within core invasion front, specifically investigating functional relevance FGFR1 FGFR2 for vitro vivo. METHOD Quantification relative levels was determined primary patient- derived lines xenograft models. Additional transcriptional profiling using RNA sequencing identified potential gene-regulatory networks involved FGFR1-driven invasion. RESULTS We found distributed across mass expressed invasive cells, while confined only. Loss FGFR1, not FGFR2, significantly reduced migration human xenografts. Comparative analysis RNA-sequencing data knockdown cells revealed a FGFR1-specific gene regulatory network associated with CONCLUSIONS Differential on functionally relevant GBM. New candidates linked FGFR1-mediated have been warrant further investigation identify regulates