Specific and Nonspecific Staining in Detecting Staphylococcal Alpha Toxin on Erythrocytes by Immunofluorescence
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چکیده
منابع مشابه
Specific and nonspecific staining in detecting staphylococcal alpha toxin on erythrocytes by immunofluorescence.
Specifically stained staphylococcal alpha toxin particles were detected on unstained and nonspecifically stained ghost membranes when rabbit erythrocytes were exposed to alpha toxin and stained with fluorescein isothiocyanate-labeled specific antitoxin. Fading of the nonspecific staining was observed and was attributed to the degradation of the membranes by alpha toxin.
متن کاملHemolysis of Rabbit Erythrocytes by Purified Staphylococcal Alpha-toxin. I. Kinetics of the Lytic Reaction.
Cooper, Louis Z. (New England Center Hospital, Boston, Mass.), Morton A. Madoff, and Louis Weinstein. Hemolysis of rabbit erythrocytes by purified staphylococcal alpha-toxin: I. Kinetics of the lytic reaction. J. Bacteriol. 87:127-135. 1964.-The hemolytic activity of purified staphylococcal alpha-lysin was found to be directly proportional to toxin concentration and inversely related to the log...
متن کاملPurification of staphylococcal alpha-toxin by adsorption chromatography on glass.
Staphylococcal alpha-toxin was purified from Staphylococcus aureus growth medium using adsorption chromatography on controlled pore glass beads. Elution of alpha-toxin from the unmodified glass surface of the beads with various anions generally followed the chaotropic series. Alpha-toxin, purified by glass bead chromatography, is composed of a single electrophoretic form, containing less than 2...
متن کاملHemolysis of Rabbit Erythrocytes by Purified Staphylococcal Alpha-toxin. Ii. Effects of Inhibitors on the Hemolytic Sequence.
Cooper, Louis Z. (New England Center Hospital, Boston, Mass.), Morton A. Madoff, and Louis Weinstein. Hemolysis of rabbit erythrocytes by purified staphylococcal alpha-toxin. II. Effect of inhibitors on the hemolytic sequence. J. Bacteriol. 87:136-144. 1964.-Study of the time course of hemolysis of rabbit erythrocytes by purified staphylococcal alpha-lysin revealed that the specific toxin-red c...
متن کاملSpecific immunofluorescence staining of Treponema pallidum in smears and tissues.
To date, tissue sections prepared from Formalin-fixed tissues have not been successfully stained with Treponema pallidum subspecies-specific antibody in a direct fluorescent-antibody assay. While current methods stain T. pallidum, they do not distinguish T. pallidum from other spirochetes such as Borrelia burgdorferi (E. F. Hunter, P. W. Greer, B. L. Swisher, A. R. Simons, C. E. Farshy, J. A. C...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1972
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.5.5.831-832.1972