SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
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چکیده
منابع مشابه
SPlinted Ligation Adapter Tagging (SPLAT), a novel library preparation method for whole genome bisulphite sequencing
Sodium bisulphite treatment of DNA combined with next generation sequencing (NGS) is a powerful combination for the interrogation of genome-wide DNA methylation profiles. Library preparation for whole genome bisulphite sequencing (WGBS) is challenging due to side effects of the bisulphite treatment, which leads to extensive DNA damage. Recently, a new generation of methods for bisulphite sequen...
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1Duke-NUS Medical School, Singapore. 2MRC London Institute of Medical Sciences (LMS), Imperial College 8 London, UK. 3Department of Statistics, Athens University of Economics and Business, Athens, GR. 4Division 9 of Brain Sciences, Faculty of Medicine, Imperial College London, UK. 5Centre for Complement and 10 Inflammation Research, Imperial College London, UK. 6Department of Statistical Scienc...
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For most sample types, the automation of RNA and DNA sample preparation workflows enables high throughput next-generation sequencing (NGS) library preparation. Greater adoption of small RNA (sRNA) sequencing has been hindered by high sample input requirements and inherent ligation side products formed during library preparation. These side products, known as adapter dimer, are very similar in s...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2016
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkw1110