Statistical Methodology in Single-Molecule Experiments
نویسندگان
چکیده
منابع مشابه
Analyzing single-molecule manipulation experiments.
Single-molecule manipulation studies can provide quantitative information about the physical properties of complex biological molecules without ensemble artifacts obscuring the measurements. We demonstrate computational techniques which aim at more fully utilizing the wealth of information contained in noisy experimental time series. The "noise" comes from multiple sources e.g., inherent therma...
متن کاملMagnetic Tweezers for Single-Molecule Experiments
Over the last decade, single-molecule techniques have proven their wide applicability in the study of processive motor proteins and other enzymes, yielding insight into their kinetics and mechanochemistry. In the context of force spectroscopy of protein–nucleic acid interactions, optical tweezers, magnetic tweezers, and atomic force microscopy have made important contributions. Advantages of ma...
متن کاملDNA Handles for Single Molecule Experiments
We have worked out a procedure for covalent binding of DNA to polystyrene microspheres for optical tweezers experiments in aqueous buffer solutions. These new coupling procedures allow preparation of DNA-microspheres in advance and storage of these beads for months. The covalent coupling of the DNA fragments facilitates the investigation of short DNA fragments (<1000 bp) and the rotationally co...
متن کاملKinetics from nonequilibrium single-molecule pulling experiments.
Mechanical forces exerted by laser tweezers or atomic force microscopes can be used to drive rare transitions in single molecules, such as unfolding of a protein or dissociation of a ligand. The phenomenological description of pulling experiments based on Bell's expression for the force-induced rupture rate is found to be inadequate when tested against computer simulations of a simple microscop...
متن کاملThermodynamics for single-molecule stretching experiments.
We show how to construct nonequilibrium thermodynamics for systems too small to be considered thermodynamically in a traditional sense. Through the use of a nonequilibrium ensemble of many replicas of the system which can be viewed as a large thermodynamic system, we discuss the validity of nonequilibrium thermodynamics relations and analyze the nature of dissipation in small systems through th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Statistical Science
سال: 2020
ISSN: 0883-4237
DOI: 10.1214/19-sts752