STED-SPIM: Stimulated Emission Depletion Improves Sheet Illumination Microscopy Resolution
نویسندگان
چکیده
منابع مشابه
Stimulated Emission Depletion (STED) Microscopy: from Theory to Practice
In recent years, various methods have been proposed to increase the resolution of fluorescence microscopy beyond the diffraction limit of light. Amongst them, Stimulated Emission Depletion (STED) microscopy, which relies on a purely physical concept of fluorescence depletion could, in theory, achieve unlimited resolution independent of the wavelength of light. In this chapter, we review the fun...
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With recent developments in microscopy, such as stimulated emission deletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we analyse how the interplay between shot noise, thermal noise, the saturation intensity of a fluorescent label, and the damage threshold of the sample changes the maximum resolution of...
متن کاملStimulated emission depletion microscopy with optical fibers
Imaging at the nanoscale and/or at remote locations holds great promise for studies in fields as disparate as the life sciences and materials sciences. One such microscopy technique, stimulated emission depletion (STED) microscopy, is one of several fluorescence based imaging techniques that offers resolution beyond the diffractionlimit. All current implementations of STED microscopy, however, ...
متن کاملTime-gating improves the spatial resolution of STED microscopy.
Stimulated-emission depletion (STED) microscopy improves image resolution by encoding additional spatial information in a second stimulated-decay channel with a spatially-varying strength. Here we demonstrate that spatial information is also encoded in the fluorophore lifetime and that this information can be used to improve the spatial resolution of STED microscopy. By solving a kinetic model ...
متن کاملResolution scaling in STED microscopy.
We undertake a comprehensive study of the inverse square root dependence of spatial resolution on the saturation factor in stimulated emission depletion (STED) microscopy and generalize it to account for various focal depletion patterns. We used an experimental platform featuring a high quality depletion pattern which results in operation close to the optimal optical performance. Its superior i...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2011
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2010.12.3748