Steroid profiling by UHPLC-MS/MS in dried blood spots collected from healthy women with and without testosterone gel administration

• Dried blood spots represent a valuable alternative to invasive venous collection. A UHPLC-MS/MS was validated for the quantitative analysis of 11 free and 8 conjugated steroids in dried spots. samples collected from healthy women during menstrual cycle 28-days testosterone gel administration were analyzed. Concentrations measured similar those serum except administration. The quantification large panel endogenous by LC–MS/MS represents powerful clinical tool screening or diagnosis diverse endocrine disorders. This approach has also demonstrated excellent sensitivity detection misuse anti-doping field, especially female athlete population. In both situations, use (DBS) could provide viable Here, evaluation DBS sampling using is described. method eleven eight then used 14 normal (control phase) followed treatment (treatment phase). Results compared with obtained matrix. Satisfactory performance all compounds terms selectivity, linearity, accuracy, precision, combined uncertainty, stability as well extraction recovery matrix effects. control phase, high correlation observed between concentrations most compounds. higher capillary than DBS, suggesting possible interference resulting contamination on finger(s) application. Steroid profiling simple efficient strategy monitoring steroid their fluctuation context steroid-related disorders, abuse anti-doping.