Strategies for Making Multimeric and Polymeric Bifunctional Protein Conjugates and Their Applications as Bioanalytical Tools

Enzymes play a central role in the detection of target molecules biotechnological fields. Most probes used are bifunctional proteins comprising enzymes and binding conjugated by chemical reactions. To create highly sensitive probe, it is essential to increase enzyme-to-binding protein ratio probe. However, if reactions required prepare probe insufficiently site-specific, may lose functionality. Genetic modifications enzyme-mediated post-translational (PTMs) can ensure site-specific conjugation proteins. They therefore promising strategies for production with high enzyme contents, i.e., polymeric Herein, we review recent advances preparation conjugates detection. We have summarized research on genetically fused enzymatically prepared proteins, will discuss potential use polymers various applications.