Structure-based engineering of environmentally sensitive fluorophores for monitoring protein-protein interactions
نویسندگان
چکیده
منابع مشابه
Structure-based engineering of environmentally sensitive fluorophores for monitoring protein-protein interactions.
Single, extrinsic, environmentally sensitive fluorophores can be used to quantitate formation of protein-protein complexes. These can be prepared semi-synthetically by covalent coupling to single cysteine mutations introduced at positions where the fluorophore is predicted to respond to formation of the complex without adversely affecting the interaction. The three-dimensional structure of a pr...
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ژورنال
عنوان ژورنال: Protein Engineering Design and Selection
سال: 1998
ISSN: 1741-0126,1741-0134
DOI: 10.1093/protein/11.9.819