STUDY ON PERIRECTAL LYMPHATICS USING BROMODEOXYURIDINE LABELED LYMPHOCYTES

Tumor distribution of bromodeoxyuridine-labeled cells is strongly dose dependent.

Bromodeoxyuridine (BrdUrd) is used extensively to measure the fraction of proliferating cells in tumors. Unlike endogenous markers of proliferation such as proliferating cell nuclear antigen (PCNA) and Ki-67, BrdUrd is exogenously administered and reaches the tumor via vasculature where it must then diffuse throughout the tissue to label S-phase cells. In this study, we examine the dose depende...

Rate of production of P32-labeled lymphocytes.

E BULK OF DATA initially accumulated regarding the lymphocyte tended to indicate that its life span was relatively short. Thus, Minot and Isaacs,’ in 1925, transfused blood from a patient with chronic lymphocytic leukemia into a patient with lymphosarcoma. The recipient’s blood showed an elevated lymphocyte count for 35 minutes before returning to normal. Yoffey,2 in 1935, showed that in the do...

Studies on the numerical changes of the lymphocytes in the efferent lymphatics with lymphogenously given antigen.

Some characteristic changes were histologically observed in the lymph node with lymphogenous administration of antigen, i.e. the expel of lymphocytes from the lymphatic matrix for early several hours just after the administration of the antigen, and transformation of the lymphatic matrix into pavement appear ance almost consisted of swollen reticulum cells. A lot of lymphocytes were seen within...

Staging of Tumiors Using Labeled Activated Lymphocytes Directed to a Tumior Specific Epitope

The Distribution of 51cr-labeled Lymphocytes into Antigen-stimulated Mice

The localization of syngeneic (51)Cr-labeled lymph node cells was investigated in CBA/J mice previously challenged with sheep erythrocytes, Salmonella H antigen, keyhole limpet hemocyanin, C57BL/6J skin, or rat skin. The effect of time, dose, and route of antigen administration on lymphocyte migration was studied in both primary and secondary responses. When the distribution pattern of lymphocy...