Synthesis and processing of kinetoplast DNA minicircles in Trypanosoma equiperdum.
نویسندگان
چکیده
منابع مشابه
Genomic organization of Trypanosoma brucei kinetoplast DNA minicircles.
The sequences of seven new Trypanosoma brucei kinetoplast DNA minicircles were obtained. A detailed comparative analysis of these sequences and those of the 18 complete kDNA minicircle sequences from T. brucei available in the database was performed. These 25 different minicircles contain 86 putative gRNA genes. The number of gRNA genes per minicircle varies from 2 to 5. In most cases, the gene...
متن کاملComplete loss of kinetoplast DNA sequences induced by ethidium bromide or by acriflavine in Trypanosoma equiperdum.
A fully kinetoplastic strain of an antigenic variant of Trypanosoma equiperdum has been made dyskinetoplastic by successive treatments of infected rats with ethidium bromide or acriflavine. After seven passages in the absence of the dye, the loss of kinetoplast DNA (kDNA) is stable and complete as shown by analytical ultracentrifugation, CsCl-dye gradients, and the reassociation kinetics of in ...
متن کاملCharacterization of the molecular components in kinetoplast- mitochondrial DNA of Trypanosoma equiperdum. Comparative study of the dyskinetoplastic and wild strains
The structure of the kinetoplast DNA of Trypanosoma equiperdum has been studied and compared to the structure of the circular mitochondrial DNA extracted from a dyskinetoplastic strain of T. equiperdum. In T. equiperdum wild type, the kinetoplast DNA constitutes approximately 6% of the total cellular DNA and is composed of approximately 3,000 supercoiled minicircles of 6.4 x 10(5) daltons and a...
متن کاملSequences of two kinetoplast DNA minicircles of Tryptanosoma brucei.
Kinetoplast DNA of Trypanosoma brucei is composed of a network of about 10,000 interlocked minicircle DNA molecules (1.0 kilobase) that are catenated with about 50 maxicircle DNA molecules (23 kilobases). Several different DNA . DNA hybridization techniques using individual minicircle DNA sequences cloned in Escherichia coli have indicated that each minicircle molecule contains about one-fourth...
متن کاملThe attachment of minicircles to kinetoplast DNA networks during replication.
Kinetoplast DNA (kDNA), the trypanosomatid mitochondrial DNA, is a network containing several thousand interlocked minicircles. During kDNA synthesis, minicircles dissociate from the network, and after replication their progeny reattach to the network periphery. Using electron microscopy autoradiography, we found that newly synthesized 3H-labeled minicircles, after short labeling periods, are c...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1989
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.9.8.3212