Synthesis of Molecularly Imprinted Polymer Nanoparticles for SARS-CoV-2 Virus Detection Using Surface Plasmon Resonance

COVID-19 caused by a SARS-CoV-2 infection was first reported from Wuhan, China, and later recognized as pandemic on March 11, 2020, the World Health Organization (WHO). Gold standard nucleic acid molecular-based testing have largely satisfied requirements of early diagnosis management this infectious disease; however, these techniques are expensive not readily available for point-of-care (POC) applications. The 21st century has emphasized that medicine is in dire need advanced, rapid, cheap diagnostic tools. Herein, we report molecularly imprinted polymer nanoparticles (MIP-NPs/nanoMIPs) plastic antibodies specific detection employing surface plasmon resonance (SPR) sensor. High-affinity MIP-NPs directed against were manufactured using solid-phase imprinting method. then characterized dynamic light scattering (DLS) atomic force microscopy (AFM) prior to their incorporation into label-free portable SPR device. Detection studied within range 104–106 PFU mL−1. demonstrated good binding affinity (KD = 0.12 pM) selectivity toward SARS-CoV-2. AFM, cyclic voltammetry, square-wave voltammetry studies revealed successful stepwise preparation A cross-reactivity test confirmed specificity For time, study demonstrates potential molecular technology conjunction with miniaturized devices particles high-affinity specificity. Such sensors could help monitor manage risks related virus contamination infections also beyond current pandemic.