Syringe loading introduces macromolecules into living mammalian cell cytosol
نویسندگان
چکیده
منابع مشابه
Syringe loading introduces macromolecules into living mammalian cell cytosol.
We describe a simple, efficient, gentle and inexpensive technique for the introduction of normally impermeant macromolecules into the cytosol of living mammalian cells growing in suspension or attached to the culturing substratum. Loading is achieved by the production of transient, survivable plasma membrane disruptions as cells are passed back and forth through a standard syringe needle or sim...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 1992
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.102.3.533