TALE proteins bind to both active and inactive chromatin

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TALE proteins bind to both active and inactive chromatin.

TALE (transcription activator-like effector) proteins can be tailored to bind to any DNA sequence of choice and thus are of immense utility for genome editing and the specific delivery of transcription activators. However, to perform these functions, they need to occupy their sites in chromatin. In the present study, we have systematically assessed TALE binding to chromatin substrates and find ...

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Histones of Genetically Active and Inactive Chromatin

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Organization of Transcriptionally Active and Inactive Chromatin

Der Aufbau des Nukleosoms als Grundbaustein des Chromatins der Eukaryontenzelle, mit Ausnahme des Dinoflagellaten-Zellkerns, wird biochemisch und elektronenmikroskopisch vorgestellt als eine Einheit eines Oktamers aus Histonen (2 X H2A, 2XH2B, 2X H3, 2X H4) und einem Betrag von etwa 150 Basenpaaren DNA. Diese Partikel sind durch einen Zwischenbereich von weiteren etwa 50 Basenpaaren DNA verbund...

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Chromatin structure of active and inactive human X chromosomes.

Nuclei from a variety of human cell lines and tissues were digested with gradually increasing levels of DNase I. The DNA was then purified, treated with restriction enzymes and subjected to Southern blot hybridization using a cloned cDNA probe to 3-phosphoglycerate kinase (PGK) a housekeeping enzyme. At relatively high levels of DNase I, a specific, slightly sensitive site in chromatin sequence...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 2014

ISSN: 0264-6021,1470-8728

DOI: 10.1042/bj20131327