Targeted and Untargeted Metabolic Profiling to Discover Bioactive Compounds in Seaweeds and Hemp Using Gas and Liquid Chromatography-Mass Spectrometry

Greenhouse gas emissions are a global problem facing the dairy/beef industry. Novel feed additives consisting of seaweeds and hemp containing bioactive compounds theorized to reduce enteric methane emissions. In this study we aimed investigate metabolic profiles brown, red green using chromatography liquid mass spectrometry. We used targeted untargeted approaches, quantifying known halomethanes phenolics, as well identifying potentially novel with anti-methanogenic properties. The main findings were: (a) Asparagopsis taxiformis contained halomethanes, high concentrations bromoform (4200 µg/g DW), six volatile halocarbons were tentatively identified; (b) no detected in other studied nor hemp; (c) lignans measured (d) numbers sulfated phenolic acids unidentified sulfuric acid-containing all seaweeds; (e) flavonoid glucosides glucuronides mainly identified (f) condensed tannin gallocatechin was Fucus sp. Using combined metabolomics approach, an overview in-depth information on secondary metabolites provided. Halomethanes have already been shown be anti-methanogenic; however, such Dictyota Sargassum also contain that may potential.