Tears contain the complement regulator CD59 as well as decay-accelerating factor (DAF)

The intrinsic complement regulator decay-accelerating factor modulates the biological response to vascular injury.

OBJECTIVE To investigate whether the presence of decay-accelerating factor (or CD55), an intrinsic complement regulator, protects against the development of vascular disease, given that complement activation can affect leukocytes and platelets. METHODS AND RESULTS Leukocyte-platelet complexes are critical for the initiation and progression of atherosclerosis and restenosis; however, the mecha...

Complement-mediated clearance of erythrocytes: mechanism and delineation of the regulatory roles of Crry and DAF. Decay-accelerating factor.

The role of complement in the pathogenesis of autoimmune hemolytic anemia (AIHA) has been controversial and may depend on a number of factors, including the affinity and isotype of the pathogenic antibodies involved. We have recently shown that mouse erythrocytes deficient in the membrane C3 regulatory protein, complement receptor 1-related gene/protein y (Crry), but not decay-accelerating fact...

Inhibition of complement activation on the surface of cells after incorporation of decay-accelerating factor (DAF) into their membranes

Decay-accelerating factor (DAF), extracted from the stroma of human erythrocytes, was purified to homogeneity and incorporated into the membrane of sheep red cell complement intermediates, where its functional properties were analyzed. Incorporation of DAF into the cell membranes was temperature dependent, took place on pronase- or trypsin-treated erythrocytes, and did not depend on prior depos...

Identification of the complement decay-accelerating factor (DAF) on epithelium and glandular cells and in body fluids

Decay-accelerating factor (DAF) is a 70 kD membrane regulatory protein that prevents the activation of autologous complement on cell surfaces. Using immunohistochemical methods and a radioimmunometric assay based on mAbs to DAF, we found large amounts of membrane-associated DAF antigen on the epithelial surface of cornea, conjunctiva, oral and gastrointestinal mucosa, exocrine glands, renal tub...

Cell-Mediated Cytolysis Complement-Mediated Cytolysis but Not NK Accelerating Factor (DAF: CD55) Inhibits Delta-Short Consensus Repeat 4-Decay