Technical aspects of circulating tumor cells (CTC) isolation using molecular sieve

Hepatocellular Carcinoma: Methods of Circulating Tumor Cells (CTC) Measurements

Circulating tumor cells (CTC) detection: clinical impact and future directions.

Recent molecular and clinical studies have shown that invasion may occur very early in tumor development, thus emphasizing the potential importance of specific and sensitive detection of circulating tumor cells (CTC) and circulating tumor microemboli (CTM). The technical challenge in this field consists of finding "rare" tumor cells (just a few CTCs mixed with the approximately 10 million leuko...

Molecular Biomarker Analyses Using Circulating Tumor Cells

BACKGROUND Evaluation of cancer biomarkers from blood could significantly enable biomarker assessment by providing a relatively non-invasive source of representative tumor material. Circulating Tumor Cells (CTCs) isolated from blood of metastatic cancer patients hold significant promise in this regard. METHODOLOGY/PRINCIPAL FINDINGS Using spiked tumor-cells we evaluated CTC capture on differe...

An Optically-induced Dielectrophoretic (odep) Microfluidic Platform for Isolation of Circulating Tumor Cells (ctcs) after Conventional Ctc Isolation Process

ABSTRACT Negative selection-based circulating tumor cells (CTCs) isolation method can harvest the CTCs in a label-free manner that is valuable for the subsequent CTC-based assays. However, such cell isolation scheme normally suffers from low CTC purity. To address the issue, the utilization of optically-induced dielectrophoretic (ODEP) force in a microfluidic chip to further purify the CTCs was...

Circulating Tumor Cells: Clinically Relevant Molecular Access Based on a Novel CTC Flow Cell

BACKGROUND Contemporary cancer diagnostics are becoming increasing reliant upon sophisticated new molecular methods for analyzing genetic information. Limiting the scope of these new technologies is the lack of adequate solid tumor tissue samples. Patients may present with tumors that are not accessible to biopsy or adequate for longitudinal monitoring. One attractive alternate source is cancer...