Technical Variations in Low-Input RNA-seq Methodologies
نویسندگان
چکیده
منابع مشابه
Technical Variations in Low-Input RNA-seq Methodologies
Recent advances in RNA-seq methodologies from limiting amounts of mRNA have facilitated the characterization of rare cell-types in various biological systems. So far, however, technical variations in these methods have not been adequately characterized, vis-à-vis sensitivity, starting with reduced levels of mRNA. Here, we generated sequencing libraries from limiting amounts of mRNA using three ...
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Recently, a number of protocols extending RNA-sequencing to the single-cell regime have been published. However, we were concerned that the additional steps to deal with such minute quantities of input sample would introduce serious biases that would make analysis of the data using existing approaches invalid. In this study, we performed a critical evaluation of several of these low-volume RNA-...
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1 Recently, a number of protocols extending RNA-sequencing to the 2 single-cell regime have been published. However, we were concerned that 3 the additional steps to deal with such minute quantities of input sam4 ple would introduce serious biases that would make analysis of the data 5 using existing approaches invalid. In this study, we performed a critical 6 evaluation of several of these low...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2014
ISSN: 2045-2322
DOI: 10.1038/srep03678