Testis-on-chip platform to study ex vivo primate spermatogenesis and endocrine dynamics

Here, we report a testis-on-chip platform for the ex vivo culture of seminiferous tubules isolated from human and non-human primate testis. Tissues are cultured in dedicated chamber with continuous perfusion via vascular-like channel. The is fabricated PDMS using 3D printed mold, after design has been optimized, e.g., barrier between COMSOL modeling revealed no direct negative impact flow on tissues applied flowrate device, shear rate remaining physiological range. Culture experiments were performed adult gender dysphoria patients prepubertal 6-month-old marmoset up to 11 9 days, respectively. First, microscopic, live imaging presence viable cell populations both types samples. Next, exposed stimulatory conditions through gonadotropins at different doses. tissue response was characterized by histological analysis their recovery device testosterone estradiol effluent. Histological observations suggested improved maintenance testicular under conditions, which similarly resulted an increase production, yet patterns low-dose high-dose stimulation. herein reported shows great promise evaluate endocrine toxic effects