The adsorption of enkephalin to porous polystyrene beads: A simple assay for enkephalin hydrolysis
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Trauma decreases leucine enkephalin hydrolysis in human plasma.
Plasma hydrolysis of leucine enkephalin was evaluated, together with several cellular immune parameters, in a homogeneous group of human subjects who had undergone severe trauma (proximal femur fracture); data obtained were compared with those obtained in an age-matched control group. In the experimental group, immediately after hospitalization, substrate hydrolysis was reduced with respect bot...
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A specific carboxypeptidase that converts enkephalin precursors into enkephalin in adrenal chromaffin granules has been purified and characterized. In the adrenal this enzyme, designated enkephalin convertase, is uniquely localized to the chromaffin granules, which contain enkephalin and precursor peptides. Enkephalin convertase is markedly stimulated by CoCl2 and inhibited by EDTA or 1,10-phen...
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Leucine-enkephalin and methionine-enkephalin are two pentapeptides with morphine like properties, naturally present in mammalian brain. They were first characterized by Hughes et al. [ 1 ] as H-TyrGlyGlyPheLeu-OH and H-TyrGlyGlyPheMet-OH respectively and found to behave as potent, although short lived opiates in a variety of pharmacological tests in vivo [2]. They do interact with the so-called...
متن کاملImmunohistochemical studies on the gastrointestinal tract using antisera to Met-enkephalin and Met-enkephalin Arg6Phe7.
Antisera specific for the C-terminus of Met-enkephalin and two of its variants isolated from adrenal medulla and brain, namely Met-enk Arg6 and Met-enk Arg6Phe7, have been used in immunohistochemical studies of the gastrointestinal tract in rat, mouse and guinea pig. Met-enk and Met-enk Arg6Phe7-like immunoreactivities were found with similar distribution in nerve cell bodies of the myenteric p...
متن کاملImmunocytochemical and ultrastructural differentiation between Met-enkephalin-, Leu-enkephalin-, and Met/Leu-enkephalin-immunoreactive neurons of feline gut.
Selective immunocytochemical procedures, applied at the light and electron microscopical level, have resulted in the identification of three types of enkephalin-immunoreactive nerves in the feline gastrointestinal tract, including neurons containing either Met- or Leu-enkephalin immunoreactivity or those simultaneously storing both Met- and Leu-enkephalin immunoreactants. The three different ty...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1977
ISSN: 0014-5793
DOI: 10.1016/0014-5793(77)80469-9