The composition of fish species shows little seasonal variation in eDNA based survey at urban streams

Biodiversity in the city is becoming important as cities are expanded. Thus, cost-efficient and accurate methodology for investigation of biota has been consistently demanded. Environmental DNA (eDNA) one emerging tools to supplement traditional survey methods improve efficiency survey. Summer (from July August) monsoon period South Korea, so it excluded from because unstable aquatic environment, safety investigators, accessibility study sites. On other hand, spring autumn March October), excluding flooding season, considered suitable times This compared fish composition distribution by season summer using eDNA identify change determine suitability seasonal at urban streams. The number reads, composition, their community structures was changed according season. total read 2.3 more October (1,419,062) than (3,202,626). In addition, when 13 families 33 species were detected, 35 detected fall. A 32 common. Three ( Acheilognathus rhombeus, Tachysurus fulvidraco, Pungtungia herzi ) identified, Hemiculter leucisculus not October. result described that rate area. Especially, appearance invasive increased 14 20 sites habitat range extended July. However, there no significant difference richness diversity except abundance among structures. revealed can changes Furthermore, based on little fluctuation appearing suggested be used complement conducting surveys regardless period.