The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus
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چکیده
منابع مشابه
Development of SYBR Green I Based Real-Time RT-PCR Assay for Specific Detection of Watermelon silver mottle Virus
Background: Watermelon silver mottle virus (WSMoV), which belongs to the genus Tospovirus, causes significant loss in Cucurbitaceae plants. Objectives: Development of a highly sensitive and reliable detection method for WSMoV. Materials and Methods: Recombinant plasmids for targeting the sequence of nucleocapsid protein gene of WSMoV were constructed. SYBR Green I real-time PCR was established...
متن کاملDevelopment of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus (D.) congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tube...
متن کاملDevelopment and validation of a SYBR green real-time PCR for the quantification of porcine circovirus
not available 687 Krakowka, S., Ringler, S.S., Arumugam, P. , McKillen, J., McIntosh, K., Hartunian, C., Hamberg, A., Rings, M., Allan, G., Ellis, J.A. (2008) Evaluation of Mycoplasma hyopneumoniae bacterins for porcine torque teno virus DNAs American Journal of Veterinary Research, 69, 1601-1607 Objective-TO determine whether commercial Mycoplasma hyopneumoniae bacterins sold for use in swine ...
متن کاملdevelopment of sybr green i based real-time rt-pcr assay for specific detection of watermelon silver mottle virus
background: watermelon silver mottle virus (wsmov), which belongs to the genus tospovirus, causes significant loss in cucurbitaceae plants. objectives: development of a highly sensitive and reliable detection method for wsmov.materials and methods: recombinant plasmids for targeting the sequence of nucleocapsid protein gene of wsmov were constructed. sybr green i real-time pcr was established a...
متن کاملIntroducing a New SYBR green Real-time PCR for Detection of SARS-CoV2 Virus Genome
Background and purpose: There are various methods for molecular detection of SARS-CoV2 genome among which, PCR-based methods are the most reliable for making diagnosis. The majority of approved PCR kits for detection of Coronavirus are based on TaqMan real-time PCR which is expensive due to incorporating fluorescent and quencher-harboring probe. The aim of this study was to design a simple and ...
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ژورنال
عنوان ژورنال: Virology Journal
سال: 2011
ISSN: 1743-422X
DOI: 10.1186/1743-422x-8-465