The Double-stranded DNA of Cauliflower Mosaic Virus

The double-stranded DNA of cauliflower mosaic virus.

Examination of the physical structure of cauliflower mosaic virus DNA by electron microscopy and ultracentrifugal analysis showed that the DNA could be isolated mainly as a circular molecule of mol. wt. 4"7 x Io 6. Nearest-neighbour frequency analysis showed that the DNA has a chemical structure which exhibits close similarities to that of the host cauliflower DNA. The relationships of this pla...

In vivo recombination of cauliflower mosaic virus DNA.

LIGATION AND RECOMBINATION OF THE DNA OF CAULIFLOWER MOSAIC VIRUS (CAMV) IS DEMONSTRATED BY THE FOLLOWING EXPERIMENTS: (i) Ligation: Different noninfectious fragments of the CaMV genome (obtained after insertion into plasmid pBR322 followed by enzymatic excision) regained infectivity when mixtures of them were used to inoculate their host. The symptom appearance was delayed by comparison with a...

A DNA polymerase activity is associated with Cauliflower Mosaic Virus.

A DNA polymerase activity is found within the Cauliflower Mosaic Virus (CaMV) particle. Analysis of the reaction product reveals that the linear form of the virion DNA is preferentially labelled. The molecular weight of the DNA polymerase as determined on an "activity gel" is 76 kDa.

Cauliflower mosaic virus isolate CMV-1.

Biochemical characterization of the helper component of Cauliflower mosaic virus.

The helper component of Cauliflower mosaic virus is encoded by viral gene II. This protein (P2) is dispensable for virus replication but required for aphid transmission. The purification of P2 has never been reported, and hence its biochemical properties are largely unknown. We produced the P2 protein via a recombinant baculovirus with a His tag fused at the N terminus. The fusion protein was p...