The Effect of Azadirachta indica leaves extract on Transforming Growth Factor-β and Tumor Necrosis Factor-α in a Plasmodium berghei infected Mice model

Malaria is a health problem for the world's population and predominantly located in tropical subtropical areas. The three countries with most malaria cases are India (58%), followed by Indonesia (20%), then Myanmar (16%). This study aims to determine effect of neem leaf extract on increasing TGF-β expression decreasing TNF-α mice infected Plasmodium berghei. In this there were four groups, namely Treatment 1 (in berghei infection without therapy). 2 treated Azadirachta indica at dose 0.25 mg / g BW). 3 = therapy leaves 0.5 4 examination elisa method immunohistochemistry. Data analysis using SEM (Structural Equation Modeling) results treatment showed decrease plasma (t 1.13; tcount 1.93; ≥ttabel 1.96) spleen (tcount 1.53; 1.45; but was an increase 1.77; 1.00; 1.96). Groups 5.13; 2.42; ≥ttable 2.00; 1.97; 2.03; 2.11; Conclusion: can spleen.
 Keywords: ethanol extract, transforming growth factor- β, tumor necrosis factor-α