The Effect of Collagen-I Coatings of 3D Printed PCL Scaffolds for Bone Replacement on Three Different Cell Types

Introduction The use of scaffolds in tissue engineering is becoming increasingly important as solutions need to be found preserve human tissues such bone or cartilage. Various factors, including cells, biomaterials, cell and culture conditions, play a crucial role engineering. vivo environment the cells exerts complex stimuli on thereby directly influencing behavior, proliferation differentiation. Therefore, create suitable replacement regeneration procedures for tissues, conditions cells’ natural should well mimicked. current research trying develop 3-dimensional (scaffolds) that can elicit appropriate cellular responses thus help body regenerate replace tissues. In this work, were printed from biomaterial polycaprolactone (PCL) 3D bioplotter. Biocompatibility testing was used determine whether Material Methods An Envisiontec bioplotter fabricate scaffolds. For better cell-scaffold interaction, coated with type-I collagen. Three different types then cultured various tests investigate biocompatibility Results Reproducible could polycaprolactone. addition, coating process collagen developed, which significantly improved interaction. showed PCL-collagen are cells. adhered surface result extensive growth observed inner part scaffolds, however, remained largely uninhabited. cytotoxicity studies, it toxicity below 20% present some experimental runs. determination compressive strength by means universal machine Z005 ZWICK according DIN EN ISO 604 resulted value 68.49 ± 0.47 MPa.