The effect of two cryopreservation methods on human sperm DNA damage
نویسندگان
چکیده
منابع مشابه
Comparisons between Two Antioxidant Butylated Hydroxytoluene and Glutathione Supplemented Cryopreservation Medium on Human Sperm DNA Integrity
Introduction: Cryopreservation of semen is routinely used in a variety of circumstances including before assisted reproduction treatments, pre- radiation or chemotherapy treatment and etc. The aim of this study was to compare the effect of Butylated hydroxytoluene (BHT) and Glutathione supplemented cryopreservation medium on sperm parameters and amount of DNA fragmentation during the freeze-tha...
متن کاملComparison of the effects of two methods of cryopreservation on testicular sperm DNA.
OBJECTIVE To assess the effects of two methods of freezing on testicular sperm DNA from subjects with obstructive azoospermia and to compare these with samples of fresh and freeze-thawed testicular sperm from fertile men. DESIGN The Comet assay was used to determine the percentage of undamaged DNA in fresh testicular sperm, testicular sperm freeze-thawed in suspension and in a biopsy sample (...
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15 صفحه اولHuman Sperm Cryopreservation: Update on Techniques, Effect on DNA Integrity, and Implications for ART
Cryopreservation of human spermatozoa-introduced in the 1960's-has been recognized as an efficient procedure for management of male fertility before therapy for malignant diseases, vasectomy or surgical infertility treatments, to store donor and partner spermatozoa before assisted reproduction treatments and to ensure the recovery of a small number of spermatozoa in severe male factor infertili...
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ژورنال
عنوان ژورنال: Cryobiology
سال: 2016
ISSN: 0011-2240
DOI: 10.1016/j.cryobiol.2016.04.004