The Endoplasmic Reticulum of Mung Bean Cotyledons
نویسندگان
چکیده
منابع مشابه
Endoplasmic Reticulum of Mung Bean Cotyledons: ACCUMULATION DURING SEED MATURATION AND CATABOLISM DURING SEEDLING GROWTH.
Homogenates of mung bean cotyledons were subjected to equilibrium density centrifugation on linear sucrose gradients and the positions of the various organelles determined by assay of marker enzymes. Measurement of phospholipid distribution on such gradients showed that the major peak of phospholipid at a density of 1.11 to 1.13 grams per cubic centimeter coincided with the position of the endo...
متن کاملProton pumping inorganic pyrophosphatase of endoplasmic reticulum-enriched vesicles from etiolated mung bean seedlings.
Endoplasmic reticulum (ER)-enriched vesicles from etiolated hypocotyls of mung bean seedlings (Vigna radiata) were successfully isolated using Ficoll gradient and two-phase (polyethylene glycol-dextran) partition. The ER-enriched vesicles contained inorganic pyrophosphate (PPi) hydrolysis and its associated proton translocating activities. Antiserum prepared against vacuolar H+-pyrophosphatase ...
متن کاملRapid degradation and limited synthesis of phospholipids in the cotyledons of mung bean seedlings.
Seedling growth of mung bean is accompanied by the rapid catabolism of the three major phospholipids in the cotyledons (phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol). The decline starts 24 hours after the beginning of imbibition and by the 4th day of growth more than 50% of the phospholipids have been catabolized. Extracts of cotyledons of 24-hour-imbibed beans contai...
متن کاملLocalization of vicilin peptidohydrolase in the cotyledons of mung bean seedlings by immunofluorescence microscopy
Vicilin peptidohydrolase, the protease that hydrolyzes the reserve proteins in the cotyledons of mung bean (Vigna radiata) seedlings, has been localized intracellularly by immunofluorescence microscopy using monospecific antibodies against the enzyme and rhodamine-coupled goat-anti-rabbit immunoglobulin G's. The enzyme can first be visualized after 3 days of seedling growth and is associated wi...
متن کاملRegulation of reserve protein metabolism in the cotyledons of mung bean seedlings.
Seedling growth in mung beans (Phaseolus aureus, Roxb.) is accompanied by the metabolism of the reserve proteins, and the appearance in the cotyledons of a proteolytic enzyme with endopeptidase activity. Enzyme activity increases 25-fold during the first 5 days of growth. Cotyledon extracts prepared from seeds imbibed for 24 hr with water do not react with rabbit endopeptidase antiserum, which ...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1980
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.66.3.390