The Generation of Transgenic Mice with <b><i>Fat1</i></b> and <b><i>Fad2</i></b> Genes that have their own Polyunsaturated Fatty Acid Biosynthetic Pathway
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چکیده
منابع مشابه
Metabolic engineering of the omega-3 long chain polyunsaturated fatty acid biosynthetic pathway into transgenic plants.
Omega-3 (ω-3) very long chain polyunsaturated fatty acids (VLC-PUFAs) such as eicosapentaenoic acid (EPA; 20:5 Δ5,8,11,14,17) and docosahexaenoic acid (DHA; 22:6 Δ4,7,10,13,16,19) have been shown to have significant roles in human health. Currently the primary dietary source of these fatty acids are marine fish; however, the increasing demand for fish and fish oil (in particular the expansion o...
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A Caenorhabditis elegans ORF encoding the presumptive condensing enzyme activity of a fatty acid elongase has been characterized functionally by heterologous expression in yeast. This ORF (F56H11. 4) shows low similarity to Saccharomyces cerevisiae genes involved in fatty acid elongation. The substrate specificity of the C. elegans enzyme indicated a preference for Delta(6)-desaturated C18 poly...
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The genes encoding Δ6 desaturase, Δ6 fatty acid elongase, and Δ5 desaturase from the alga, Phaeodactylum tricornutum, were co-expressed in Arabidopsis thaliana under the control of the Brassica napus napin promoter to produce arachidonic acid (ARA; 20:4 Δ 8, 11, ) and eicosapentaenoic acid (EPA; 20:5 Δ 8, 11, 14, ). ARA and EPA accumulated up to 0.5% and 0.05% of total fatty acids, respectively...
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The nutritional and pharmaceutical values of long-chain polyunsaturated fatty acids (LC-PUFAs) such as arachidonic, eicosapentaenoic and docosahexaenoic acids have been well recognized. These LC-PUFAs are physiologically important compounds in bacteria and eukaryotes. Although little is known about the biosynthetic mechanisms and functions of LC-PUFAs in bacteria compared to those in higher org...
متن کاملExpression of fatty acid and lipid biosynthetic genes.
An assessment of the transcript abundance of several fatty acid and lipid biosynthetic genes has been made. Total RNA was extracted from embryos at different stages of development and mRNA was analysed by quantitative Northern blots and used to probe cDNA arrays. Central fatty acid synthase components were present at different steady-state levels, but at constant ratios throughout development; ...
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ژورنال
عنوان ژورنال: Cellular Physiology and Biochemistry
سال: 2013
ISSN: 1015-8987,1421-9778
DOI: 10.1159/000354456