The Mechanism of Transcription Stalling under Torsion
نویسندگان
چکیده
منابع مشابه
Transcription under torsion.
In cells, RNA polymerase (RNAP) must transcribe supercoiled DNA, whose torsional state is constantly changing, but how RNAP deals with DNA supercoiling remains elusive. We report direct measurements of individual Escherichia coli RNAPs as they transcribed supercoiled DNA. We found that a resisting torque slowed RNAP and increased its pause frequency and duration. RNAP was able to generate 11 ± ...
متن کاملPolymerase Stalling during Replication, Transcription and Translation
Replication, transcription, and translation stress all lead to stalling of their respective polymerases (DNA polymerase, RNA polymerase, and the ribosome), and the cell must respond to these events in order to preserve macromolecular integrity. In response to replication stress such as DNA damage, the cell activates a checkpoint and promotes repair or bypass at the lesion. Transcriptional stres...
متن کاملthe impact of phonetic transcription on iranian intermediate efl learners’ pronunciation accuracy
این تحقیق به منظور یافتن تاثیر آموزش آوا نگاری انگلیسی بر روی صحت تلفظ زبان آموزان سطح متوسط ایرانی صورت گرفت.برای دستیابی به دو گروه متجانس, از110 زبان آموز در حال یادگیری زبان در اموزشگاه ها ازمون opt گرفته شد. بنابراین 40 نفر از این افراد انتخاب و به دو گروه 20 تایی تقسیم شدند.درنهایت این نتیجه حاصل شد که آموزش آوانگاری انگلیسی تاثیر مثبتی بر صحت تلفظ زبان آموزان سطح متوسط ایرانی داشته است. ...
Theoretical analysis of transcription process with polymerase stalling.
Experimental evidence shows that in gene transcription RNA polymerase has the possibility to be stalled at a certain position of the transcription template. This may be due to the template damage or protein barriers. Once stalled, polymerase may backtrack along the template to the previous nucleotide to wait for the repair of the damaged site, simply bypass the barrier or damaged site and conse...
متن کاملReversible stalling of transcription elongation complexes by high pressure.
We have investigated the effect of high hydrostatic pressure on the stability of RNA polymerase molecules during transcription. RNA polymerase molecules participating in stalled or active ternary transcribing complexes do not dissociate from the template DNA and nascent RNA at pressures up to 180 MPa. A lower limit for the free energy of stabilization of an elongating ternary complex relative t...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2015
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2014.11.2938