The miniF plasmid C protein: sequence, purification and DNA binding
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چکیده
منابع مشابه
Purification and characterization of nuclear factor III (origin recognition protein C), a sequence-specific DNA binding protein required for efficient initiation of adenovirus DNA replication.
Nuclear factor III (NF-III, origin recognition protein C) is a cellular DNA binding protein that has high affinity for a DNA sequence contained within the adenovirus origin of DNA replication. We have purified NF-III more than 760-fold from HeLa nuclear extracts by a combination of conventional methods and DNA recognition site affinity chromatography. The NF-III polypeptide has an apparent mole...
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The affinity chromatography procedure described in this unit uses DNA containing specific recognition sites for the desired protein that has been covalently linked to a solid support. Preparation of a DNA affinity resin, including cyanogen bromide (CNBr) activation of the agarose support, is described, and an alternate protocol provides a method to couple DNA to commercially available CNBr-acti...
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A novel and efficient method has been developed for isolation of correctly digested DNA fragments without the use of classic size-dependent electrophoretic separation methods. To achieve this, DNA fragments are end-labelled by haptens. After specific endonuclease digestion of the hapten-labelled DNA, the DNA is incubated with a protein that specifically binds to the hapten. The incubation mixtu...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1986
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/14.24.9699