THE NON-ENZYMATIC HYDROLYSIS OF p-NITROPHENYL PHOSPHATE
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چکیده
منابع مشابه
A menadione-dependent enzymatic hydrolysis of p-nitrophenyl phosphate.
Sonic extracts of Clostridium sticklandii contain an enzyme that hydrolyzes p-nitrophenyl phosphate to yield equivalent amounts of p-nitrophenol and orthophosphate. A large number of common phosphate esters is not decomposed. The most striking property of the bacterial enzyme is its dependence on both menadione (2-methyl-1,4-napthoquinone or vitamin Ka) and sulfhydryl compounds for activity, Th...
متن کاملPotassium ion-activated hydrolysis of p-nitrophenyl phosphate in pancreatic islet-cell membranes.
Hydrolysis of p-nitrophenyl phosphate was measured in a fraction enriched in plasma membranes from pancreatic islets of non-inbred ob/ob mice. Hydrolysis was stimulated by K+ (10mM) in the pH range 5--10; a small peak of K+-induced activation was observed between pH7.5 and 8. Both the K+-induced activation and the hydrolysis in the absence of K+ were Mg2+-dependent; maximum activation was obtai...
متن کاملCatalytic activity of enzyme in water/organic cosolvent mixtures for the hydrolysis of p-nitrophenyl acetate and p-nitrophenyl benzoate
The dependence of the catalytic activities of α-chymotrypsin on the concentration of organic cosolvents (acetonitrile, dimethyl sulfoxide, dimethyl formamide, ethylene glycol, methanol, ethanol, propan-2-ol and tert-butanol) in mixed aqueous media has been studied towards hydrolysis of p-nitrophenyl acetate and p-nitrophenyl benzoate using cetyltriphenylphosphonium bromide surfactant at pH 7.75...
متن کاملLipoprotein Lipase-catalyzed Hydrolysis of p-Nitrophenyl Butyrate
The mechanism of action of bovine milk lipoprotein lipase (LpL) was studied with a water-soluble substrate of p-nitrophenylbutyrate (PNPB). The calculated maximal velocity ( Vmm) and Michaelis constant (Km) values at 37 "C were 2.0 pmol of p-nitrophenol released/min/ mg of LpL and 0.52 mM, respectively. The addition of phospholipid vesicles enhanced the rate of PNPB hydrolysis by LpL. In the pr...
متن کاملTHE inhibition of chymotrypsin by diethyl p-nitrophenyl phosphate.
The alkyl fluorophosphonates were first shown in 1940 (Adrian, Feldberg & Kilby, 1947) to be very powerful inhibitors of cholinesterase, and for a time they were thought to be quite specific for this enzyme. Later, other esterases, such as human milk lipase and liver esterase (Webb, 1948) and citrus acetylesterase (Jansen, Nutting & Balls, 1947) were also shown to be inhibited, although not to ...
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ژورنال
عنوان ژورنال: Canadian Journal of Chemistry
سال: 1958
ISSN: 0008-4042,1480-3291
DOI: 10.1139/v58-096